rabbit anti human emmprin cd147 antibody  (Boster Bio)

Journal: Molecular medicine reports

Article Title: NS-398 promotes pancreatic cancer cell invasion by CD147 and MMP-2 via the activation of P38.

doi: 10.3892/mmr.2016.4783

Figure Lengend Snippet: Figure 3. NS‑398 induces the expression of CD147 and MMP‑2 via the activation of P38 in pancreatic cancer cells. (A) PANC‑1 cells were treated with 0, 5, 10, 25, 50 and 100 µM NS‑398 for 24 h. The expression levels of P‑P38, CD147 and MMP‑2 were analyzed using western blotting. (B) PANC‑1 cells were pretreated with 20 µM SB‑203580 for 2 h, following which the cells were incubated with or without 50 µM NS‑398 for 24 h. Western blot analysis was used to detect the expression levels of P‑P38, CD147 and MMP‑2. (C and D) The densitometric analysis for the data shown in (A) and (B) for P‑P38, CD147 and MMP‑2 is shown. P‑, phosphorylated; CD147, cluster of differentiation 147; MMP‑2, matrix metalloproteinase‑2.

Article Snippet: The cells were cultured for a further 24 h at 37 ̊C, following which the total protein extracts (see the ʻwestern blot analysisʼ section below) were isolated and analyzed using rabbit anti-human CD147 (cat. no. 13287; 1:1,000) and rabbit anti‐human β-actin (cat. no. 4970; 1:1,000) antibodies (both obtained from Cell Signaling Technology, Danvers, MA, USA) for immunoblotting.

Techniques: Expressing, Activation Assay, Western Blot, Incubation

Journal: Molecular medicine reports

Article Title: NS-398 promotes pancreatic cancer cell invasion by CD147 and MMP-2 via the activation of P38.

doi: 10.3892/mmr.2016.4783

Figure Lengend Snippet: Figure 4. CD147 siRNA inhibits the expression of MMP‑2 induced by NS‑398. siRNAs targeted specifically against CD147 or negative control siRNA (NC) were transiently transfected into PANC‑1 cells. At 24 h post‑transfection, the cells were incubated with or without 50 µM NS‑398 for 24 h. (A) Expression levels of CD147, MMP‑2, P‑P38 and P38 were detected using western blotting, with β‑actin as a loading control. (B) The densitometric analysis of the western blot in (A) for P‑P38, CD147 and MMP‑2 is shown. (C) Cell viability was evaluated using a Cell Counting Kit‑8 assay. (D) Invasiveness of PANC‑1 was determined using an inverted microscope, under which number of invasive cells were counted. Data are presented as the mean ± standard deviation. CD147, cluster of differentiation 147; siRNA, small interfering RNA; Con, control; NC, negative control; OD, optical density.

Article Snippet: The cells were cultured for a further 24 h at 37 ̊C, following which the total protein extracts (see the ʻwestern blot analysisʼ section below) were isolated and analyzed using rabbit anti-human CD147 (cat. no. 13287; 1:1,000) and rabbit anti‐human β-actin (cat. no. 4970; 1:1,000) antibodies (both obtained from Cell Signaling Technology, Danvers, MA, USA) for immunoblotting.

Techniques: Expressing, Negative Control, Transfection, Incubation, Western Blot, Control, CCK-8 Assay, Inverted Microscopy, Standard Deviation, Small Interfering RNA

Journal: Oncology Letters

Article Title: Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

doi: 10.3892/ol.2012.981

Figure Lengend Snippet: Correlation between EMMPRIN/CD147 expression and clinicopathological characteristics.

Article Snippet: Subsequently, the membrane was blocked with 5% non-fat dry milk in TBST [10 mmol/l Tris-HCl (pH 7.5), 150 mmol/l NaCl and 0.05% Tween-20] at room temperature for 1 h. The proteins were probed with rabbit anti-human EMMPRIN/CD147 antibodies (1:1,000; Boster, Wuhan, China) or control anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH, Sigma) at 4°C overnight.

Techniques: Expressing